P-734 Blastocysts derived from tripronuclear zygote including small pronuclear zygote have high diploid and euploid rate with NGS and SNP analysis.

Abstract Study question What is diploid and euploid frequency of blastocysts derived from tripronuclear zygote (3PN) including a small pronucleus (2.1PN) with NGS SNP analysis? Summary answer Diploid rate the 3PN 2.1PN was 91.3%, in which euploid, aneuploid mosaic embryos were 18.7%, 61.3% 11.3%, respectively. known already A thought to be produced when two sperm enter into same oocyte during vitro fertilization (IVF) or whenthe second polar body not extruded on both IVF intracytoplasmic injection (ICSI). It has been reported that observed around 3-10%, are usually discarded by reason for genetic risks triploid. However, some studies blastocyst and/or (defined as PN < 20μm) contained normal preimplantation testing, healthy babies born these embryos. design, size, duration We conducted cross-sectional study between July 2019 June 2022 single reproduction center. included 80 this generated zygotes zygotes. Of these, 71 ICSI 9 IVF. These assessed chromosomal ploidy next generation sequencing (NGS) nucleotide polymorphism (SNP) analysis embryonic development monitored time-lapse imaging system. Participants/materials, setting, methods Average age women 39.2 years (22-47 years). The smallest pronuclear diameter who provided 12.7±2.8μm (8.5-19.7μm) average. divided inner cell mass (ICM) trophectoderm (TE) using laser system under inverted microscope, followed SNP. t-test performed analyze differences triploid diploid. Statistical significance defined P 0.05. All participants written informed consent, Institutional Review Board approval obtained. Main results role chance blastocysts, 64 (80%) able ICM TE. In terms diploid, TE completely concordant, although 15.6% discordancy ages patients triploid, aneuploid, 41.1±5.1 years, 36.0±6.3 40.6±3.5 35.2±3.6 patients’ significantly higher than those (P = 0.015 0.001, respectively). Mean triploids diploids 15.2±4.1μm (8.5-18.9μm), embryos, comparable mean pronucleus. There no insemination (IVF ICSI), abnormal blastomere division, developmental speed, grade, Limitations, reasons caution Since cytogenetics causal association incidence (including 2.1PN) postnatal findings extra micro unknown. need assess longitudinal-study large sample size embryo transfer PGT-A. Wider implications revealed had high analysis. Therefore, could used after chromosome analyses. Trial registration number Not applicable